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Cryo-electron microscopy (cryo-EM) is a three-dimensional (3D) averaging technique that makes use of two-dimensional (2D) images of biological macromolecules preserved in a thin layer of vitreous ice. Recent advances in the field have facilitated the evolution of cryo-EM towards atomic resolution, and the technique provides 3D maps with detailed description of biological macromolecules. Data acquisition...
Cryo-electron microscopy (Cryo-EM) data acquisition on modern transmission electron microscopes (TEM) is the first step during the single-particle analysis workflow. Importantly, the demand for large number of two dimensional images requires reliable and efficient automation of image data collection. We present a novel control scheme for automated cryo-EM data collection that monitors the quality...
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