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The catalytic subunit of protein phosphatase 1 (PP1c) was purified from an alfalfa (Medicago sativa) microcallus cell culture. The preparation was inhibited by rabbit muscle inhibitor-2 and okadaic acid and had a molecular mass of 35 kDa. Five distinct cDNAs termed MsPP1α, -β, -γ, -δ, and -ε were cloned from aM. sativasomatic embryo library. MsPP1α was identical to a cDNA reported earlier [A. Pay,...
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