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There remains an urgent need for rapid diagnostic methods that can evaluate antibiotic resistance for pathogenic bacteria in order to deliver targeted antibiotic treatments. Toward this end, we present a rapid and integrated single-cell biosensing platform, termed dropFAST, for bacterial growth detection and antimicrobial susceptibility assessment. DropFAST utilizes a rapid resazurin-based fluorescent...
We report a miniaturized droplet magnetofluidic assay system on a cartridge smaller than 1 cubic inch for automated purification, amplification and melt analysis of RNA targets. This platform demonstrates quantification and confirmation of nucleic acid targets through postamplification analysis with comparable performance to benchtop reverse transcriptase PCR (RT-PCR) assays. Implementation of precise...
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