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An immunochromatographic assay for the detection of hepatitis B surface antigen (HBsAg) was developed using Fe3O4 nanoparticles as signal materials. Anti-HBsAg antibodies were labeled with carboxylated Fe3O4 particles with an average diameter of 153.6 nm, and an immunochromatography strip was constructed. The detection capability of the assay developed here is 1 ng/mL HBsAg.
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