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DOI: 10.1002/pmic.201800433
Chemical proteomics enables comprehensive profiling of small molecules in complex proteomes. In this study, fluorescence‐triggered two‐dimensional chromatography based on the application of a bromocoumarin tag facilitates the isolation of small molecule‐conjugated peptides prior to mass spectrometry. Profiling of a cysteinome with the use of a bromocoumarin tag demonstrates...
Chemical proteomics enables comprehensive profiling of small molecules in complex proteomes. A critical component to understand the interactome of a small molecule is the precise location on a protein where the interaction takes place. Several approaches have been developed that take advantage of bio‐orthogonal chemistry and subsequent enrichment steps to isolate peptides modified by small molecules...
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