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The survival states of Saccharomyces cerevisiae cells after being treated by pulsed electric field (PEF), intact, sublethally injured or dead, were quantitatively investigated in this paper. A selective culture medium with critical NaCl concentration of 4.25% (w/v) was developed firstly and then used to identify and quantify the sublethally injured cells (SICs) from dead and intact cells. Results...
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