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Single-nucleotide polymorphism analysis based on fabrication of the abasic site (AP site) in the thiolated DNA duplexes is demonstrated. The AP site allows an electrochemical indicator to bind to target nucleotides accompanied by electrochemical signaling and to detect a specific nucleobase of a target nucleic acid. A mixed self-assembled monolayer of thiolated AP site-containing probe DNA is hybridized...
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