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In native tissues, the majority of medically important membrane proteins is only present at low concentrations, making their overexpression in recombinant systems a prerequisite for structural studies. Here, we explore the commonly used eukaryotic expression systems—yeast, baculovirus/insect cells (Sf9) and Semliki Forest Virus (SFV)/mammalian cells—for the expression of seven different eukaryotic...
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