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The oxygen binding behaviour of hemocyanins from Crustacea is regulated by small organic compounds such as urate and l-lactate. We investigated the binding characteristics of urate and the related compound caffeine to the 2x6-meric hemocyanin of A. leptodactylus under fully oxygenated conditions employing isothermal titration calorimetry (ITC). An analysis of urate and caffeine binding based on a...
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