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This study was aimed to investigate the effects of SB203580, the specific p38 mitogen-activated protein (MAP) kinase inhibitor, on cardiac myocyte survival and secretion of cytokines in an in vitro model of hypoxia and burn serum challenge. Results demonstrated that hypoxia and burn serum induced a persistent activation of p38 MAP kinase in primary cultured neonatal rat cardiomyocytes during the 12h...
The present study was designed to assess the effects of induced heat shock protein 70 (HSP70) on intestinal injury after severe burn. Wistar rats were randomly divided into four groups: control group, burn group (B group), sodium arsenite pretreatment group (SA group), and sodium arsenite+quercetin pretreatment group (SA+Qu group). Plasma endotoxin and d-lactic acid content were determined at 3, 6,...
The aim of this study was to investigate the changes of myocardial Gsα mRNA expression and ATPase and the effects of Panax notoginseng saponins (PNS) on that after burns in rats. Wistar rats were exposed to a 95 o C water bath for 10s to produce 30% TBSA skin-full-thickness scalds. Myocardial Gsα mRNA level, cAMP content and adenyl cyclase (AC) activities were determined with dot blotting...
Objective: To investigate the change of myocardial mitochondrial Ca 2+ transport and its mechanism in the early stage after burns. Methods: Forty-eight Wistar rats were randomized into a normal control group (n=8) and a burns group (n=40). The rats of the burns group were given a 30%TBSA full-thickness. Myocardial mitochondria were isolated from normal and scalded rats which were sacrificed...
Objective: To explore the role of Ca 2+ in the damage to myocardial mitochondrial respiratory function in the early stage after severe burns. Methods: An experimental model of 30%TBSA full-thickness skin scalding was reproduced in rats. Myocardial mitochondria were isolated from control and burned rats in the 1st, 3rd, 6th, 12th and 24th hour post-burn. The mitochondrial respiratory function,...
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