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The variable cyanide-sensitivity of the iron-containing alcohol dehydrogenase isoenzyme (ADH II) of the ethanol-producing bacterium Zymomonas mobilis was studied. In aerobically grown permeabilized cells, cyanide caused gradual inhibition of ADH II, which was largely prevented by externally added NADH. Cyanide-sensitivity of ADH II was highest in cells grown under conditions of vigorous aeration,...
The use of a newly synthesized fluorescent probe, ABM (conditional name), to determine changes of membrane composition (fluidity), biomass yield, ethanol productivity, and yield of Zymomonas mobilis under anaerobic conditions was investigated. A strong correlation between ABM spectral characteristics in cell suspension and all obtained parameters was observed. It was concluded that application of...
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