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Capillary electrophoresis (CE) with electrochemiluminescence (ECL) detection was used to explore the kinetics of the enzymatic reaction. The different effects of reaction conditions including the concentration of Mn 2+ , incubation temperature and pH on prolidase (PLD, EC 3.4.13.9) activity in erythrocyte lysates against three different substrates, Gly-Pro, Val-Pro and Leu-Pro were investigated...
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