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Compared to robotic injection of suspended cells (e.g., embryos and oocytes), fewer attempts were made to automate the injection of adherent cells (e.g., cancer cells and cardiomyocytes) due to their smaller size, highly irregular morphology, small thickness (a few micrometers thick), and large variations in thickness across cells. This paper presents a robotic system for automated microinjection...
This paper presents the use of electrodeformation as a method for single cell mechanical characterization. Cells were placed between two microelectrodes with a rectangular AC electric field applied and cell deformation recorded. Numerical simulations were performed to model cell electrodeformation based on the Maxwell stress tensor formulation in which effects of cell electrical property variations...
This paper presents an approach to optimize the zebrafish-embryo microinjection process by selecting suitable speed trajectories for the motion of the micropipette, with the objective of minimizing the risk of damaging the embryo during the microinjection process. A viscoelastic model of a zebrafish embryo is developed based on experimental data. The speed optimization problem is formulated based...
This paper describes the development of an explicit force control system for exerting a suitable force on the zebrafish chorion while simultaneously measuring its impedance, and presents results demonstrating that the application of an external force leads to significant change in the impedance of a zebrafish embryo. These results offer support for a plausible explanation that activities of pore canals...
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