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To investigate B cell epitopes on the VP3 of infectious bursal disease virus (IBDV), the VP3 gene of IBDV were subcloned into the pET28a vector to construct expression vector of pETVP3. The recombinant VP3 (rVP3) was expressed in E. coli BL21(DE3) by inducing with IPTG. The expressed VP3 protein was recognized specifically by anti-IBDV antisera in western blot assays. According to antigenic structure...
Metabolic pathways constructed from sequenced genomes or transcriptomes can not only help predict metabolic phenotypes and discover enzyme but also provide an essential context for understanding comprehensive experimental data and the systematic workings of a cell. In the present study we compared the metabolic pathways of two Actinobacillus pleuropneumoniae strains by Pathway Tools, a web-based pathway...
Alpha-fetoprotein (AFP) is a major serum protein (Mr, =70,000) synthesized during fetal life. Reappearance of AFP in adult serum often signals pathological conditions, particularly hepatocarcinomas and teratocarcinomas. To obtain monoclonal antibodies against AFP for further study of the structure and biological function of AFP, a novel method for making hybridoma cell lines producing monoclonal antibodies...
Determination of protein structural class is a quite meaningful topic in protein science, because a priori knowledge of a protein structural class can provide useful information about its overall structure. The results of most previous studies used high homologous dataset with four structural classes should not be perceived as reliable, because the sequence homology has very significant impact on...
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