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CD4+CD25+ regulatory T cells (Tregs) mediate tolerance towards self antigens and prevent the development of autoimmunity. Treg function is typically evaluated by the ability to suppress proliferation and cytokine production of co-cultured CD4+CD25− T cells in Treg suppression assays. Purified Tregs are often obtained using the “Regulatory T Cell isolation kit” from Miltenyi Biotech. Separation can...
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