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Aequorin is a Ca 2+ -binding photoprotein and consists of an apoprotein (apoaequorin) and a 2-peroxide of coelenterazine. Eight new coelenterazine analogues modified at the C2-position were synthesized and incorporated into recombinant apoaequorin with O 2 to yield different semisynthetic aequorins. The luminescence properties and the sensitivity to Ca 2+ of these semisynthetic...
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