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Objective: Oxidative stress is a candidate mechanism for ethanol neuropathology in fetal alcohol spectrum disorders. Our overall objective was to investigate the hypothesis that ethanol neurotoxicity involves production of reactive oxygen species (ROS) with negative downstream consequences for mitochondrial membrane potential and neuron survival. Methods: We use P4 cerebellar granule neurons (CGNs)...
Response surface methodology was used to optimize the extraction parameters of EPS produced by Agaricus bisporus MJ-0811 in submerged culture. The optimal levels for ethanol concentration (85%, v/v), pH (8) and precipitation time (22h) were determined, and EPS production was estimated at 2.71g/L. The actual yield of EPS under these conditions was 2.69g/L. In addition, the antioxidant activity of EPS...
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