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Because of the need for antibodies in our studies involving the third component of complement in Bufo arenarum, we performed a simple procedure to purify C 3 from B. arenarum serum to use as antigen in the preparation of the antiserum. The strategy was based on the well-known ability of C 3 to bind to zymosan (Zy), a yeast cell wall extract comprised of polysaccharides. The Zy-bound...
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