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A new compound, 2,5-di-[2-(4-hydroxy-phenyl)ethylene]-terephthalonitrile (DHPEPN), was synthesized. The interaction between bovine serum albumin (BSA) and DHPEPN in Tris–HCl buffer solution (pH 7.4) was investigated using fluorescence and UV–vis absorption spectroscopy. The mechanism of BSA fluorescence quenched by DHPEPN is discussed according to the Stern–Volmer equation. The binding constant and...
A precise, simple, new spectrofluorimetry method is proposed for determination of trace antimony which is based on the reaction between potassium periodate and the new type fluorescent reagent 3-o-chlorophenyl-5-(2′- arsenoxylphenylazo) rhodanine (2ClRAAP). The possible mechanism is proposed. The fluorescence intensity is investigated to be sharply enhanced by the oxidation of 3-o-chlorophenyl-5-(2′-arsenoxylphenylazo)...
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