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Background: Several methods have been established to detect the JAK2 V617F mutation, a frequent event involved in the pathogenesis of myeloproliferative neoplasms (MPNs). High‐resolution melt (HRM) analysis is a newly established technique without the requirement of any gel‐based post‐PCR handling. Methods: An asymmetric PCR with unlabeled specific probe was developed and combined to HRM analysis...
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