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The human MHC region has been shown to be associated with a wide range of diseases.The recent advancement in the next generation sequencing (NGS) technology has definitely helped in increasing the resolution (up to 4-field) of HLA genotyping. However, it remains a challenge in interpreting the results from NGS platform due to biases including systematic sequencing error by sequencing platform, difference...
In the present work we established a rapid, cost-effective and high-throughput method for genotyping using a multiplexed microsphere-based suspension array platform - Luminex R xMAP ™ which enabled us to analyze 3 SNPs in the MBL2 gene promoter and 5′ UTR, and 3 coding SNPs exon 1 haplotypes, associated with different levels of MBL2 expression. Using this system MBL2 diversity in four...
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