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CHO-K1 cells were synchronized at the G 1 /S border by mitotic shake-off and aphidicolin incubation. Pulse-labeling with tritium was done at 30 min, 2 or 5 h into the S-phase, with chase incubations for different times in non-radioactive medium. The cells were subjected to neutral microelectrophoresis to extend the DNA into ''comets,'' after which the label was visualized through autoradiography...
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