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Backward walking, a form of knee rehabilitation shown to protect knee structures, has been predicted to maintain cardiorespiratory fitness. The purpose of this study was to investigate the motor learning effects on oxygen uptake (VO2), heart rate (HR), and ratings of perceived exertion (RPE) during a backward-walking habituation program. Eight males completed baseline measures of VO2, HR, and RPE...
Under standard growth conditions, E. coli transformed with the high-level expression vector pMON5525 produces recombinant DMAPP/AMP transferase in inactive, insoluble complexes. We have produced large amounts of active, soluble protein by growing and inducing the cells under osmotic stress in the presence of sorbitol and glycyl betaine. This caused an increase of up to 427-fold in the active yield,...
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