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Optimization of a matrix-assisted laser desorption ionization-ion mobility-surface-induced dissociation-o-time-of-flight mass spectrometer for peptide sequencing is discussed. Surface-induced dissociation (SID) spectra obtained by using stainless steel, Au grids, and fluorinated self-assembled monolayers (F-SAM) on Au are compared. The F-SAM surfaces yield similar fragment ions to those obtained using...
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