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Dithiothreitol effectively separated the laminae densae and the laminae fibroreticulares of the basement membranes of oral mucosal epithelia as it does the epidermis and dermis. Dithiothreitol-separated epithelial basement membranes of palate, tongue, and lip were immunoreacted with colloidal gold-conjugated anti-type VII collagen antibody. By transmission electron microscopy, gold particles were...
It is known that the gaps between epithelium and the underlying connective tissue usually occur between the epithelial cells and the basement membrane, resulting in exposure of the cellular surface of the lamina densa. After dithiothreitol separation, the epithelia of oral mucosa, gingiva, and tongue were mechanically peeled off from the underlying connective tissues. This treatment severed the connections...
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