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A new optical device has been developed to allow the observation of microorganisms during a high pressure treatment up to 700 MPa. To measure cell volume variation during the high pressure application, an image analysis system was connected with the light microscope. With this device, growth of Saccharomyces cerevisiae was studied at moderate pressure (10 MPa) through the observation of individual...
The variation rate of the temperature increase was found to have a great effect on the viability of Saccharomyces cerevisiae subjected to heat perturbations between 25° C and 50° C. A low intensity of the increase rate of temperature could maintain an important viability of the cells (about 34% of the initial population) with regard to the corresponding viability (about 1%) observed after a sudden...
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