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Renewable alternatives for petroleum-derived chemicals are achievable through biosynthetic production. In this work, Pichia stipitis was engineered for itaconate production from xylose. The heterologous cis-aconitate decarboxylase from Aspergillus terreus was overexpressed with codon optimization, and 52mg/L itaconate was produced by the initial engineered strain TJUIA01. To improve itaconate production,...
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