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To determine whether oocyte activation using a combination of calcium ionophore A23187 (A23187) with puromycin could salvage human unfertilized oocytes after ICSI.One hundred and thirteen discarded unfertilized oocytes 20–68h after ICSI were assigned to four groups: ICSI 20-h group, ICSI 44-h group, ICSI 68-h group and control. All unfertilized oocytes were exposed to A23187 (5μM) for 5min and subsequently...
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