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Our previous study revealed that the Hill coefficient of Escherichia coli methylglyoxal synthase (EMGS) is higher than what we have calculated for MGS from Thermus sp. GH5 (TMGS). Amino acid sequence alignment of EMGS and TMGS shows that key residues of allosteric pathways in EMGS exist in TMGS as well, except Arg150 which plays a crucial role in forming a salt bridge with Asp20 in the neighboring...
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