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N-acetyl chitobiose was produced by partial hydrolysis of the marine biopolymer chitin using industrially bulk-produced enzyme preparations. Pepsin showed the highest chitinolytic activity. Pepsin treatment of chitin at pH 5.4 at 44°C yielded 71.5% of chitobiose, 19% of N-acetyl glucosamine and 9.5% chitotriose after incubation during 24h. Suspensions of amorphous chitin and phosphoric decrystallized...
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