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Microtiter plates are suitable for screening and process development of most microorganisms. They are currently the container of choice for high‐throughput and small‐scale microbial culture, but require optimization for specific work. In this research, a novel type of microtiter plate was developed using computational fluid dynamics (CFD) technology. The new plate provided high oxygen supply and optimal...
Glucosamine‐6‐phosphate N‐acetyltransferase (GNA1) that catalyzes acetyl transfer from acetyl‐coenzyme A to glucosamine‐6‐phosphate (GlcN‐6P), and glutamine‐fructose‐6‐phosphate aminotransferase (GlmS) that catalyzes the formation of GlcN‐6P from fructose‐6‐phosphate (Fru‐6P), are two key enzymes in Bacillus subtilis for the bioproduction of N‐acetylglucosamine (GlcNAc), a nutraceutical that has...
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