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We have developed a highly sensitive and selective on-line biosensor for detecting the reduced form of nicotinamide adenine dinucleotide (NADH) produced by the enzymatic reactions of dehydrogenases with various substrates such as glutamate. The sensor consists of a glassy carbon electrode modified with an osmium-polyvinylpyridine-based bottom layer containing horseradish peroxidase, and a bovine serum...
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