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Objective To improve the operational stability and reusability of an epoxide hydrolase (EH) for the biosynthesis of optically active epoxides. Results A covalently immobilization strategy was employed to improve the stability of Agrobacteriumradiobacter EH by using ethylenediamine (EDA)-functionalised epoxy resin LX-1000EP as carrier. Under the optimal conditions, the activity recovery of immobilized...
Objectives The broad host range of pseudorabies virus (PRV) and large capacity for foreign DNA make it a promising vector for the development of vaccines and agents of gene therapy. Results We show that up to 100 % viral gene disrupting efficiency was achieved by simple co-transfection of the purified PRV genomes with the clustered regularly-interspaced, short palindromic repeats (CRISPR)/CRISPR-associated...
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