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We have studied the equilibrium unfolding and the kinetics of folding and unfolding of an antibody scFv fragment devoid of cis-prolines. An anti-GCN4 scFv fragment carrying a V L lambda domain, obtained by ribosome display, served as the model system together with an engineered destabilized mutant in V H carrying the R66K exchange. Kinetic and equilibrium unfolding experiments indicate...
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