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The objective of this study was to investigate the effect of serum on in vitro maturation of Korean Native Cattle(KNC) oocytes. Oocytes were collected by slicing the ovaries of slaughtered Korean Native Cows with a surgical blade and matured in TCM199 supplemented with 10% FBS, 5%, 10%, and 20% steer serum(SS), 5%, 10% and 20% porcine serum(PS), 5%, 10% and 20% chicken serum(CS) in 5% CO 2 ...
The objectives of this study were to evaluate the effect of uterine or oviductal epithelial cells on development of Korean Native Cattle(KNC) oocytes fertilized in vitro. Oocytes were collected from ovaries of slaughtered Korean Native Cows and matured in TCM199 with granulosa cells supplemented with 10% FBS, 5μg/ml FSH, 10 IU hCG and 1μg/ml estradiol-17 β for 24 hrs. Fertilized ova were co-cultured(39°C,...
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