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A “two-in-one” bilayer immunoaffinity column (IAC) coupled in tandem was prepared for the highly selective purification of olaquindox (OLA) and its major metabolite, 3-methyl-quinoxaline-2-carboxylic acid (MQCA), and their determinations were carried out with the developed high-performance liquid chromatography–tandem mass spectrometry (LC–MS/MS) method. The prepared highly selective two-in-one IAC...
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