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Purpose The aim of the study is to evaluate Fourier-transform infrared spectroscopy (FTIR) as an analytical tool for high-concentrated protein formulations. Methods FTIR is used to determine the melting temperature (Tm (FTIR)) of various proteins, such as bovine serum albumin (BSA), immunoglobulin (IgG1), β-lactoglobulin (β-LG), and lysozyme (HEWL), at different protein concentrations (5–100 mg/mL),...
The goal of the study was to analyze the impact of human serum albumin (HSA) quality (stabilized or nonstabilized HSA), the addition of NaCl, and the HSA stabilizers Naoctanoate and Na-N-acetyltryptophanate on the freezing behavior of mannitol-HSA formulations. The focus was on crystallization, Tg' (glass transition temperature of the maximally freeze-concentrated phase), and Tc (collapse temperature)...
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