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This study identified the widely used T7 in vitro transcription system as a major source of artifact in the tiling array data from nine eukaryotic genomes. The most affected probes contained a sequence motif complementary to the +1 to +9 initial transcribed sequence (ITS) of the T7-(dT) 24 primer. The abundance of 5′ ITS cRNA fragments produced during target preparation was sufficient to drive...
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