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Nature relies on a wide range of enzymes with specific biocatalytic roles to carry out much of the chemistry needed to sustain life. Enzymes catalyze the interconversion of a vast array of molecules with high specificity—from molecular nitrogen fixation to the synthesis of highly specialized hormones and quorum‐sensing molecules. Ever increasing emphasis on renewable sources for energy and waste minimization...
This paper discusses predictive models for quantifying the outcome of DNA recombination employed in directed evolution experiments for the generation of novel enzymes. Specifically, predictive models are outlined for (i) tracking the DNA fragment size distribution after random fragmentation and subsequent assembly into genes of full length and (ii) estimating the fraction of the assembled full length...
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