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β1,3-N-Acetylglucosaminyltransferase-7 (β3Gn-T7) has been identified as an anti-migration factor for a lung cancer cell line but its enzymatic activity has not yet been characterized. Here we show that β3Gn-T7 efficiently acts on keratan sulfate-related glycans including Galβ1->4(SO 3- ->6)GlcNAcβ1->3Galβ1->4(SO 3- ->6)GlcNAc (L2L2), while lacto-N-tetraose...
6-Sulfo-sialyl Lewis X structure is attributable to recognition between lymphocytes and high endothelial venules. However, the biosynthetic pathway still remains unclear. We found that a β-galactosyltransferase (βGalT) in human colorectal mucosa preferentially acts on GlcNAc-6-O-sulfate (6S-GN). 6S-GN:β4GalT was partially purified by UDP-hexanolamine-Sepharose and asialo-agalacto-ovomucin-Sepharose...
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