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In this study, thermoreversible phase transitions of high (HMP) and low (LMP) melting point agarose gels were investigated by using the UV–vis and fluorescence spectroscopy techniques. Pyranine was added to the aqueous solution of agarose as a fluorescence-probe. Transmitted light (Itr) and fluorescence emission (Ifl) intensities from the gel samples with different agarose concentrations were monitored...
The effect of agarose content on thermal phase transitions of the agarose gels was investigated by using Steady State Fluorescence (SSF) method. Scattered light, Isc and fluorescence intensity, Ifl were monitored against temperature during heating and cooling processes to investigate phase transitions. Two regions were observed during the heating and cooling processes. At the high temperature...
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