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Purpose: Structural biology of membrane proteins is not fully developed because of the difficulty of their crystallization. However, bacteriorhodopsin(bR) forms exceptionally excellent two dimensional crystals even in plasma membrane of Halobacterium salinarium. To obtain 2D crystals of sensory opsin(sR) as an example of the membrane proteins, a chimerical protein with bR was investigated.Methods:...
Purpose: To study dynamic characters and functions of bio-molecules at the single molecular level.Methods: Total internal reflection fluorescence microscopy was refined to directly visualize single fluorescent dye molecules in solution. Nanometer-scale motions of single molecules were detected by optical trapping nanometry.Results: We succeeded in visualizing single fluorescent dye molecules in aqueous...
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