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In order to study proteoglycan retention and accumulation, we optimized a chondrocyte cell culture system in which isolated bovine articular chondrocytes accumulate extracellular matrix to form a continuous layer of cartilagenous tissue. The tissue can attain a thickness of up to 110 μm by 35 days. The cells synthesize large keratan sulfate containing proteoglycans and type II collagen indicating...
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