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The initiation of Ca2+ release from internal stores in the egg is a hallmark of egg activation. In sea urchins, PLCγ activity is necessary for the production of IP3, which leads to the initial rise in Ca2+. To examine the possible function of a tyrosine kinase in activating PLCγ at fertilization, sea urchin eggs were treated with the specific Src kinase inhibitor PP1 or microinjected with recombinant...
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