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To discuss the mechanism of the apoptosis induced by n-butanol extract from Capparis spionosa L. (CSBE) in human heptocarcinoma cell Line HepG-2, Laser Scanning Confocal Technology and Flow Cytometry were used to investigat the changes of mitochondrial membrane potential and Ca2+ levels in HepG-2 cells. The changes of mitochondria membrane potential induced by CSBE on the HepG-2 cells were studied...
The purpose of this article was to observe interaction between drug and cancer cell with fluorescence probes and molecular imaging technology. In order to study the effect of cell growth and apoptosis morphology of Human gastric adenocarcinoma SGC-7901 induced by n-butanol extract from Capparis spionosa L. (CSBE), laser scanning microscopy was employed to observe apoptosis imaging. In addition, the...
The purpose of this article was to observe interaction between drug and cancer cell with fluorescence probes and molecular imaging technology. In order to study the effect of cell growth and apoptosis morphology of Human gastric adenocarcinoma SGC-7901 induced by n-butanol extract from Capparis spionosa L. (CSBE), laser scanning microscopy was employed to observe apoptosis imaging. In addition, the...
The purpose of this article was to investigate the apoptosis effect of the n-butanol extract from Capparis spinosa L. (CSBE) on human Gastric Cancer cells SGC-7901. MTT assay was used to measure the inhibitory rate of CSBE Morphological changes of apoptosis were observed with fluorescence microscope. Flow cytometry was employed to analyse the apoptotic rate of CSBE on SGC-7901 cells. Laser confocal...
This article studied whether genetic toxicity of toluene diisocyanate was reversible. Kunming mice were exposed to TDI by respiratory tract for 14 days in 1/8LC50, 1/4LC50 and 1/2 LC50 dosage and then were feed normally for 14 days. The chromosomal aberrations and the content of RNA & DNA were detected by optical microscope and laser scanning confocal microscope. In 1/4LC50 and 1/2 LC50 group,...
Laser scanning confoncal technology was used to study relationship between apoptosis and changes of Ca2+ induced by CSA (Capparis spinosa L. total alkaloid, CSA) on human heptocarcinoma cell HepG-2. Killing effects of CSA on human heptocarcinoma cell HepG-2 was measured by MTT method, while morphological observation on HepG-2 cells was completed by fluorescence microscope. Apoptosis of HepG-2 cells...
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