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Large polysaccharide molecules composing the glycocalyx have been shown to prevent cell adhesion. However, this process was not observed microscopically. Terbium labeling, combined with a new quantitative imaging method based on electron energy loss spectroscopy, allowed specific glycocalyx staining with excellent contrast. Image analysis enabled us to compare glycocalyx structure in free membrane...
Electron Energy-Loss Near Edge Structure (ELNES) mapping has been performed by using an energy-filtering TEM and the recently developed image-spectrum acquisition technique. The performance of the method in terms of spatial and energy resolution was evaluated by using a Zeiss 902 EFTEM and a specific software for this application. The experimental model was calcite material (CaCO 3 ) deposited...
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