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Improving the specificity of α‐cyclodextrin glucanotransferase is a significant issue in the field of α‐cyclodextrin production. In this study, a constructed Y167H mutant α‐cyclodextrin glucanotransferase with enhanced α‐cyclodextrin specificity was successfully expressed and purified. Single crystals were grown using PEG 4000 as a precipitating agent by the hanging‐drop vapour‐diffusion method at...
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