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Antimycin A, an inhibitor of electron transport in mitochondria, has been used as reactive oxygen species (ROS) generator in the biological system. Here, we investigated the in vitro effect of antimycin A on apoptosis in As4.1 juxtaglomerular cells. Antimycin A efficiently induced apoptosis in As4.1 cells as evidenced by flow cytometric detection of sub-G 1 DNA content, annexin V binding assay...
We investigated an involvement of ROS, such as H 2 O 2 and O 2− and GSH in the As4.1 cell death by antimycin A and examined whether ROS scavengers rescue antimycin A-induced As4.1 cell death and its mechanism. Levels of intracellular H 2 O 2 and O 2− were markedly increased in antimycin A-treated cells. Antimycin A reduced the intracellular...
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