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Levan is a fructan polymer that offers a variety of applications in the chemical, health, cosmetic and food industries. Most of the levan applications depend on levan molecular weight, which in turn depends on the source of the synthesizing enzyme and/or on reaction conditions. Here we demonstrate that in the particular case of levansucrase from Bacillus subtilis 168, enzyme concentration is also...
A full-length cDNA encoding for 1-SST in Agave tequilana Weber var. azul. was isolated, cloned and expressed in Pichia pastoris. The heterologous protein, with a molecular mass of 75kDa, shows identity with different plant fructosyltransferases and invertases, which belong to the glycoside hydrolase 32 family. When sucrose was used as substrate, only the transference products 1-kestose and glucose...
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