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INTRODUCTION: Mesenchymal Stem Cells (MSC) possess ability to release cytokines and growth factors that suppress immune responses and stimulate tissue regeneration. Wharton’s Jelly-derived MSC (WJ-MSC) in the addition to the strong adjuvant properties are characterized by low immunogenicity. AIM(S): The aim of this study is to verify immunomodulatory properties of WJ‑MSC after TNFα and IFNγ stimulation...
INTRODUCTION: MSC-based therapy is becoming more and more common treatment of various diseases, albeit still as an experimental approach. According to present‑day literature, the therapeutic effects of transplanted cells would not be ascribed to their differentiation, trans‑differentiation or repopulation but rather to their paracrine effect on damaged tissue. This way of treatment can be initiated...
While the rapid development of stem cell-based therapies are nowadays running, the reliable protocols leading to safe and effective way for cell isolation, expansion and commitment in vitro according to distinguished therapeutic purposes still need more consensus and standardization. In order to obtain particular disease-committed therapeutic cells we have screened various culture conditions, especially...
Here we provide a comprehensive data on the unique features of mesenchymal stem cells (MSCs) which makes them feasible and preferred candidate for cell-based therapy in neurological clinic. From this point of view the most important features of these cells are: (1) availability from autologous sources independently from age of patient; (2) extensive expansion in vitro; (3) immunomodulatory "bystander"...
The NG2-positive cells are the oligodendrocyte precursors, which, when terminally differentiated, are capable of myelinating the central nervous system. There is however an ever-growing list of evidences that NG2 cells actually possess an intrinsic neurogenic potential and they are capable of neuronal differentiation in response to environmental stimuli. To address the question, we have established...
In the context of cell therapy, the epigenetic status of core stemness transcription factor (STF) genes regulating the cell proliferation/differentiation program is of primary interest. Our results confirmed that in vitro differentiation of the umbilical- cord-blood-derived-neural-stem-cells (HUCB-NSC) coincides with the progressive down-regulation of Oct3/4 and Nanog gene expression. Consistently...
The oxygen tension is an important factor modulating cell fate and developmental decisions. There are evidences that HIFs (Hypoxia Inducible Factors) family is implicated in the regulation of pluripotency and differentiation genes. The goal of this study is to compare the influence of close to physiological oxygen conditions (5%) to atmospheric oxygen tension on differentiation process and pluripotent...
Mesenchymal stem cells (MSC) exert unique ability to differentiate into various cells of mesodermal origin. These properties place MSC as a very promising source of cells for regenerative medicine and tissue engineering. Recently, it has been shown that experimental transplantation of MSC improves a variety of neurological dysfunctions. Bone marrow (BM) represents the mostly exploited source of human...
Human umbilical cord blood (HUCB) has been established as a promising source of hematopoietic as well as various non- hematopoietic stem cell populations. This offers numerous advantages of HUCB stem/progenitor cells for therapies, however in vitro conditions that contribute to long term propagation of proliferating undifferentiated cells have not yet been established. Here we evaluate culture conditions...
The aim of the study was to evaluate therapeutic effectiveness of intra-arterial infusion of human umbilical cord blood (HUCB) derived cells at different stages of their neural conversion. Freshly isolated mononuclear cells (D-0), neurally directed progenitors (D-3) and neural-like stem cells derived from umbilical cord blood (NSC) were compared. Focal brain damage was induced in rats by stereotactic...
Many types of neural progenitors from various sources have been evaluated for therapy of CNS disorders. Prerequisite for success in cell therapy is the ability for transplanted cells to reach appropriate target such as stroke lesion. We have established neural stem cell line from human umbilical cord blood neural stem (HUCB-NSC). In the present study we evaluated migratory capabilities of cells (HUCB-NSC)...
Physical interactions of cells with the adhesive substrates of the microenvironment as well as the presence of the soluble growth factors are important for the proliferation capacity of neural stem cells. We have used biofunctionalized surface domains microcontact printed with either synthetic polyaminoacid poly-L-lysine or extracellular matrix (ECM) component such as fibronectin, to study the proliferation...
In CNS, transient global or severe focal ischemia leads to devastating irreversible cellular losses and functional impairments of the nervous tissue. Stem/progenitor cells have been considered the salvage therapy. However, besides decision on the cells source, stage of their development and proliferation, the limited survival of any implanted exogenous cells in the host tissue is one of the major...
Mesenchymal stem cell (MSC) transplantation offers new therapeutic avenue for neurological diseases, however limitted survival of exogenous cells in the host brain is a major setback. The aim of this study was to evaluate the efficacy of using biodegradable scaffolds or Wharton jelly implants contained MSC after their transplantation into rat brain.Materials and methods: Adult Wistar rats were transplanted...
Mesenchymal stem cells (MSC) emerged as promising candidates for therapeutic applications in regenerative medicine and tissue engineering. The ability of MSC to differentiate into multiple different cells of mesodermal origin has offered therapeutic tool for the treatment leukaemias and other malignant diseases. Recently, MSC have been shown to ameliorate a variety of neurological dysfunction. The...
The oligodendrocyte precursors exhibit many features of neural stem cells and constitute the abundant population of dividing progenitors in the young and adult brain. A question arises if their commitment and development could be modulated by either local tissue-specific or neuropathological signals. The aim of our study was to evaluate the effect of distinct microenvironments (provided by either...
The NG2 cells are the oligodendrocyte precursors that terminally differentiated are capable for myelination of central nervous system (CNS). They exhibit many features of neural stem cells and constitute the abundant population of dividing progenitors in the young and adult brain. A question arises if their commitment could be modulated by local tissue-specific or neuropathological signals. The aim...
Mesenchymal stem cells (MSC) are of clinical interest because of their potential use in autologous transplantation. The ability of MSC to differentiate into multiple different cells of mesodermal origin has offered therapeutic tool for the treatment of hematopoietic malignancies and graft versus host disease. Recently, MSC have been shown to ameliorate a variety of neurological dysfunction. This effect...
Over the last decade a large number of studies explored the use of cord-blood-derived stem cells for treatment of neurological disorders. Despite of some positive preclinical results low survival of transplanted cells was noticed in the host brain. It seems that transplantation of the donor cells in their own milieu might be more effective due to the natural cell-cell contact and the presence of growth...
Introduction: Neurological disorders are the most common cause of serious disability and have a major impact on financial healthrelated burden to society. Most of them are definitely associated with cell death: sudden or chronic. Conventional treatment methods yield disappointing results. Thus the discoveries in stem cell biology have fueled the interest in cell-based therapeutical approach. Based...
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